Search results for "ion-exchange chromatography"
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Improving Process-Scale Chromatography
Developments are also being made in ion-exchange chromatography, primarily in the area of capacity improvements to reduce cost and improve ease of use, according to Lynch. Thermo Fisher has continued …
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Process Chromatography Selection for Downstream Processing Applications
Becerra-Arteaga (MilliporeSigma): Ion-exchange chromatography is most commonly used to separate product- and process-related impurities in the purification of biologics that are not mAbs. In cases whe…
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Process Chromatography: Continuous Optimization
Improved resin chemistries and customized separation solutions are enabling more efficient separations.
By Cynthia A. Challener
Cell-culture and fermentation are high-…
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Ion Exchange Chromatography
Ion-exchange chromatography (IEC) is widely used for profiling the charge heterogeneity of proteins, yet despite good resolving power and robustness, ionic strength-bas…
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Mechanistic Modeling of Preparative Ion-Exchange Chromatography
For example, for the commonly used ion-exchange chromatography, pH, ion concentration, gradient, and stationary phase can affect the recovery step as well as the resulting product quality.
For com…
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Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
…ermined using a high-throughput process development (HTPD) 96-well plate prefilled with a strong cation-exchange chromatography medium.
Dynamic binding capacity (DBC) was assessed using high-thro…
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How Can A Chromatography Bead Free Up Your Time?
Have you ever considered how a resin bead in your chromatography column can affect your ion exchange (IEX) chromatography run time?
Read this blog and learn how you can optimize the time spen…
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Asked and Answered: Your Questions on Ion-Exchange Chromatography
Check out what questions your fellow protein purifiers have related to ion-exchange chromatography.
The questions are organized in by chief concern, and include buffers, experimental design, L…
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Evolution of the Monoclonal Antibody Purification Platform
Various authors have described cation-exchange chromatography as a capture step in mAb purification (8-11). Though it may not be as efficient as Protein A in terms of selectivity of HCP, it can be use…
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Trends in Downstream Bioprocessing
Downstream processing involves the recovery and purification of protein therapeutics for biopharmaceuticals, vaccines, and other biologics. It involves the removal of insoluble cell debris and parti…